minimal cmv promoter (Addgene inc)
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Addgene inc
minimal cmv promoter
Minimal Cmv Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/minimal+cmv+promoter/pLenti+CMVtight+eGFP+Puro+(w771-1)+(Plasmid+%2326431)/pmc12802846-169-32-35
Average 94 stars, based on 14 article reviews
Minimal Cmv Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/minimal+cmv+promoter/pLenti+CMVtight+eGFP+Puro+(w771-1)+(Plasmid+%2326431)/pmc12802846-169-32-35
Average 94 stars, based on 14 article reviews
minimal cmv promoter - by Bioz Stars,
2026-09
94/100 stars
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Sequencing:Article Title: In Situ synNotch-Programmed Astrocytes Sense and Attenuate Neuronal Apoptosis. Article Snippet: The promoters on the 5′-terminal of synNotch receptor genes were a CMV promoter with a CMV enhancer or a GfaABC1D promoter followed by a Promega Chimeric Intron (Addgene plasmid, #100889). .. The response element downstream of the synNotch receptor contained an inducible promoter covering five copies of the Gal4 DBD targeting the UAS (5′-GGAGCACTGTCCTCCGAACG-3′) or seven copies of the TetR targeting the TRE sequence (5′-TCCCTATCAGTGATAGAGA-3′), followed by a Article Title: In Situ synNotch-Programmed Astrocytes Sense and Attenuate Neuronal Apoptosis Article Snippet: The promoters on the 5′-terminal of synNotch receptor genes were a CMV promoter with a CMV enhancer or a GfaABC1D promoter followed by a Promega Chimeric Intron (Addgene plasmid, #100889). .. The response element downstream of the synNotch receptor contained an inducible promoter covering five copies of the Gal4 DBD targeting the UAS (5′-GGAGCACTGTCCTCCGAACG-3′) or seven copies of the TetR targeting the TRE sequence (5′-TCCCTATCAGTGATAGAGA-3′), followed by a Plasmid Preparation:Article Title: In Situ synNotch-Programmed Astrocytes Sense and Attenuate Neuronal Apoptosis. Article Snippet: The promoters on the 5′-terminal of synNotch receptor genes were a CMV promoter with a CMV enhancer or a GfaABC1D promoter followed by a Promega Chimeric Intron (Addgene plasmid, #100889). .. The response element downstream of the synNotch receptor contained an inducible promoter covering five copies of the Gal4 DBD targeting the UAS (5′-GGAGCACTGTCCTCCGAACG-3′) or seven copies of the TetR targeting the TRE sequence (5′-TCCCTATCAGTGATAGAGA-3′), followed by a Article Title: A programmable genetic platform for engineering noninvasive biosensors. Article Snippet: All final plasmids were assembled via Gibson assembly and verified by whole- plasmid nanopore sequencing (Plasmidsaurus). .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible Article Title: A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200) and Aqp1 were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: In Situ synNotch-Programmed Astrocytes Sense and Attenuate Neuronal Apoptosis Article Snippet: The promoters on the 5′-terminal of synNotch receptor genes were a CMV promoter with a CMV enhancer or a GfaABC1D promoter followed by a Promega Chimeric Intron (Addgene plasmid, #100889). .. The response element downstream of the synNotch receptor contained an inducible promoter covering five copies of the Gal4 DBD targeting the UAS (5′-GGAGCACTGTCCTCCGAACG-3′) or seven copies of the TetR targeting the TRE sequence (5′-TCCCTATCAGTGATAGAGA-3′), followed by a Article Title: A Dual-gene Reporter-amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange. Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200), Aqp1, and Aqp1-FKBP12DD were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A programmable genetic platform for engineering noninvasive biosensors Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible Amplification:Article Title: A programmable genetic platform for engineering noninvasive biosensors. Article Snippet: All final plasmids were assembled via Gibson assembly and verified by whole- plasmid nanopore sequencing (Plasmidsaurus). .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible Article Title: Engineering ligand stabilized aquaporin reporters for magnetic resonance imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences - DHFR (Addgene 29326), ER (Addgene 37261), miniIAA7 (Addgene 129721), SMASh (Addgene 68853), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2X Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N-terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible Article Title: A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200) and Aqp1 were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A Dual-gene Reporter-amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange. Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200), Aqp1, and Aqp1-FKBP12DD were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A programmable genetic platform for engineering noninvasive biosensors Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible Article Title: Destabilized reporters for background-subtracted, chemically-gated, and multiplexed deep-tissue imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences – DHFR-DD (Addgene 29326), ER-DD (Addgene 37261), miniIAA7 (Addgene 129721), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2× Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N -terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible Clone Assay:Article Title: A programmable genetic platform for engineering noninvasive biosensors. Article Snippet: All final plasmids were assembled via Gibson assembly and verified by whole- plasmid nanopore sequencing (Plasmidsaurus). .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible Article Title: Engineering ligand stabilized aquaporin reporters for magnetic resonance imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences - DHFR (Addgene 29326), ER (Addgene 37261), miniIAA7 (Addgene 129721), SMASh (Addgene 68853), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2X Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N-terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible Article Title: A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200) and Aqp1 were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A Dual-gene Reporter-amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange. Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200), Aqp1, and Aqp1-FKBP12DD were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A programmable genetic platform for engineering noninvasive biosensors Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible Article Title: Destabilized reporters for background-subtracted, chemically-gated, and multiplexed deep-tissue imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences – DHFR-DD (Addgene 29326), ER-DD (Addgene 37261), miniIAA7 (Addgene 129721), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2× Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N -terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible Control:Article Title: A programmable genetic platform for engineering noninvasive biosensors. Article Snippet: All final plasmids were assembled via Gibson assembly and verified by whole- plasmid nanopore sequencing (Plasmidsaurus). .. The genes of interest were amplified using Q5 High- Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline- inducible Article Title: Engineering ligand stabilized aquaporin reporters for magnetic resonance imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences - DHFR (Addgene 29326), ER (Addgene 37261), miniIAA7 (Addgene 129721), SMASh (Addgene 68853), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2X Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N-terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible Article Title: A dual-gene reporter-amplifier architecture for enhancing the sensitivity of molecular MRI by water exchange Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200) and Aqp1 were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A Dual-gene Reporter-amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange. Article Snippet: .. The DNA sequences encoding Oatp1b3 (Addgene plasmid #132200), Aqp1, and Aqp1-FKBP12DD were amplified using Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA) and cloned by Gibson assembly in a lentiviral transfer vector under the control of a constitutive promoter, EF1α (Addgene plasmid #60058) or a doxycycline-inducible Article Title: A programmable genetic platform for engineering noninvasive biosensors Article Snippet: .. The genes of interest were amplified using Q5 High-Fidelity DNA Polymerase and cloned into a lentiviral transfer plasmid under the control of either a constitutive promoter EF1α (Addgene, #60058) or a doxycycline-inducible Article Title: Destabilized reporters for background-subtracted, chemically-gated, and multiplexed deep-tissue imaging Article Snippet: Type F immersion oil was purchased from Leica Microsystems (Deerfield, IL, USA). .. Plasmids harboring the various degron sequences – DHFR-DD (Addgene 29326), ER-DD (Addgene 37261), miniIAA7 (Addgene 129721), and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2× Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N -terminus of the aquaporin-1 reporter (Aqp1) under the control of either a constitutive promoter, EF1α (Addgene 60058) or a doxycycline-inducible |
